DNA isolation and amplification of Dryobalanops oblongifolia DREY and Dryobalanops lanceolata BURCK

Dryobalanops belong to a member of Dipterocarpaceae, which has high economic value. Due illegal harvesting for its wood, the population tends decrease every year, so rapid conservation effort was needed. D. oblongifolia and lanceolata were important members dryobalanops, have listed as endangered (EN), according IUCN. This study aims provide information on DNA isolation amplification process. data needed basic further research, such genetic diversity, genetics, phylogeny studies, or biogeography aspects. performed using CTAB method (Cetyl Trimethyl Ammonium Bromide). The process with three barcode markers those trnL-trnF, rbcL, matK. results showed quite good yield. Amplified all primers successfully an annealing temperature 490C matK, 500C 550 C rbcL.